The artist has donated the full amount of his £25k Mercury Prize prize money to the MVT, which operates to preserve the United Kingdom's small music venues.
The star was awarded the cheque on mid-October as his new release People Watching was chosen as the top album of the last 12 months.
He has chosen to donate the funds to the trust, in recognition of the crucial part grassroots venues fulfilled in his initial steps.
"I personally would not have achieved what I am doing today if it weren't for all the performances I played around the North East, and beyond, when I was starting out," stated Fender. "These spaces are renowned, but they are facing challenges."
Beginning in 2023, more than 150 of these locations have permanently closed – roughly 16 percent of the whole industry in the UK.
In the last year, major artists including Pulp, Coldplay, the singer Katy Perry and Ed Sheeran have all backed the Music Venues Trust in its initiatives to help venues survive, by adding a minor surcharge to their ticket costs, which goes to help local stages.
Sam Fender also participated, raising more than £100,000 on his recent tour to support thirty-eight local spots across England, Scotland, and Wales.
These funds supported locations that were dealing with possible closure due to difficulties stemming from flooding, fire incidents and family tragedies, as well as permit problems, legal disputes and sound issues.
Other venues received financial assistance in upgrading infrastructure and gear that positively impacted artists and concertgoers.
"The idea that funds from performances in major spaces helps the independent spots, where it begins for musicians like me, is just logical," Fender has said.
Fender is far from the only musician to give his Mercury Prize winnings to worthy causes.
When the band received the prize in 1996 for their release the album, lead singer Cocker declared that the band would donate their winnings to the organization War Child.
During 2002, artist she divided her award between a few organizations, including the NSPCC and a Sickle Cell charity.
"I also gave a £1,000 to Highgate Newtown, to their gymnastics class, because I trained in gymnastics when I was younger and they required updated gear," Ms Dynamite explained to outlets.
Two years ago, Ezra Collective gave their funds to the community center that nurtured their band, as well as other grassroots music organisations.
And 1994 winners M People gave their award to a MS organization after a close contact was diagnosed the illness.
"Receiving the award was quite enough," stated vocalist Heather Small. "The cash was the cherry on top but we didn't require the extra, because we had the main prize. So our winning affected somebody else's life."
Recently, the trust revealed it had saved two local spots in the South East, by bringing them into community ownership.
One venue in Southampton and The Croft in that location were bought under the Own Our Venues program, which is supported by Arts Council England and enthusiasts who acquire "stakes" in the buildings.
